Nacl on Peroxidase Activity

NaCl Effects on Peroxidase Activity My essay was to see if adding NaCl to theme would have both effects on peroxidase activity. The materials that were used in this audition were pH 7 buffer(DI water), peroxidase, NaCl, guaiacol and heat content hydrogen peroxide added in that order. Blanks were created for each NaCl immersion, 0%, 5%, 7. 5% and 10%. each cuvette had . 5ml of pH 7 buffer, 1ml of peroxidase, . 02ml guaiacol for the experimental cuvettes and 0ml of guaiacol for the lily-white cuvettes, . 2ml of hydrogen peroxide and . ml of dissimilar concentrated NaCl in each cuvette. When it came to put down data for my experiment, I placed the cuvette in the spectrometer, which was set to 500nm, later adding the guaiacol and hydrogen peroxide right before. I recorded the absorbance every 15 seconds for 3 minutes. I ran the experiment twice for preciseness and got the average of the two tests. I and so entered the data into JMP and do two graphs mavin to see the human race between the concentration of NaCl and activity and the another(prenominal) to see how saltiness affect enzyme activity.The commencement exercise graph alone required the clip and the mean absorbance. To nark the second graph, I had to find beat X, the time where reply starts to slow down, which in my case was 90 seconds. The axes for the graph were magazine X as the y-axis and NaCl concentrations as the x-axis. The graphs showed me that thither is a relationship between NaCl concentrations and peroxidase activity. to a greater extent the concentration of NaCl, the meteoric the reaction occurs.